Key result
Thoracic aortic dissection shows distinct transcriptome profiling with over 1,300 differentially expressed lncRNAs and mRNAs.
Why the study?
The potential core genes involved in the pathogenesis of human thoracic aortic dissection were not well defined through transcriptome profiling of lncRNAs and mRNAs.
Case-Control (n=12)
No
Transcriptome profiling reveals significant alterations in lncRNA and mRNA expression in thoracic aortic dissection, highlighting potential core genes involved in its pathogenesis.
Answers are grounded in this paper and cite their sources.
Differential lncRNA/mRNA profiles in TAD may indicate biomarkers; hypothesis-generating and should not yet change practice.
The aim of the present study was to determine the potential core genes in the pathogenesis of human thoracic aortic dissection (TAD) by analyzing microarray profiles of long non‑coding (lnc)‑RNAs between TAD and normal thoracic aorta (NTA). The differentially expressed lncRNA profiles of the aorta tissues between TAD patients (TAD group, n=6) and age‑matched donors with aortic diseases (NTA group, n=6) were analyzed by lncRNAs microarray. Gene ontology (GO), pathway and network analyses were used to further investigate candidate lncRNAs and mRNAs. Differentially expressed lncRNAs and mRNAs were validated by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). In total, the present study identified 765 lncRNAs and 619 mRNAs with differential expression between TAD and NTA (fold change >2.0, P<0.01). GO analysis demonstrated that the differentially upregulated lncRNAs are associated with cell differentiation, homeostasis, cell growth and angiogenesis. Kyoto Encyclopedia of Gene and Genomes pathway analysis demonstrated that the differentially downregulated lncRNAs are mainly associated with arrhythmogenic right ventricular cardiomyopathy, hypertrophic cardiomyopathy and dilated cardiomyopathy. To reduce the lncRNAs for further investigation and to enrich those potentially involved in TAD, a total of 16 candidate lncRNAs with a significant expression (fold change >4, P<0.01) were selected, that were associated with an annotated protein‑coding gene through the GO term and scientific literatures. Then a set of significantly expressed lncRNAs [purinergic receptor P2X7 (P2RX7), hypoxia inducing factor (HIF)‑1A‑AS2, AX746823, RP11‑69I8.3 and RP11‑536K7.5) and the corresponding mRNAs (P2RX7, cyclin dependent kinase inhibitor 2B, HIF‑1A, runt‑related transcription factor 1, connective tissue growth factor and interleukin 2 receptor a chain] were confirmed using RT‑qPCR. The present study revealed that the expression profiles of lncRNAs and mRNAs in aorta tissues from TAD were significantly altered. These results may provide important insights into the pathogenesis of TAD disease.
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Li et al. (2018) conducted a case-control in Thoracic aortic dissection (n=12). Thoracic aortic dissection vs. Normal thoracic aorta was evaluated on Differential expression of lncRNAs and mRNAs (fold change >2.0, p=<0.01). Transcriptome profiling identified 765 lncRNAs and 619 mRNAs with significant differential expression (fold change >2.0) between thoracic aortic dissection and normal thoracic aorta tissues.
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