Key result
hERG channel activation shows greater temperature sensitivity than inactivation, better described by Eyring than Q10 models.
Why the study?
Temperature-dependent changes in hERG channel kinetics are commonly represented with Q10 coefficients or an Eyring relationship, but the validity of these representations requires assessment.
Population
Chinese hamster ovary cells overexpressing hERG1a
Comparison
hERG kinetics characterized at five temperatures between 25 and 37°C
Design
Preclinical study using a 15-second information-rich optimized protocol on a 384-well automated patch-clamp platform
Authors
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May refine preclinical hERG assay design; leaves open human translation for arrhythmia risk assessment.
A 15-second optimized protocol demonstrates that hERG channel activation is highly temperature-sensitive and better modeled by a generalized Eyring relationship than standard Q10 coefficients.
Lei et al. (2019) studied hERG channel kinetics. Temperature variation (25 to 37°C) was evaluated on Temperature dependence of hERG gating. Activation of the hERG channel is far more temperature sensitive than inactivation, and its temperature dependency broadly follows a generalized Eyring relationship rather than Q10 coefficients.
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