Key result
A new electrophysiological protocol combined with mathematical modeling of drug-binding kinetics provided quantitative predictions of action potential modulation that were closer to experimental data.
Population
HERG channels stably expressed in Chinese hamster ovary cells
Comparison
New electrophysiological protocol delineating… vs Simpler model based on conductance reduction
Design
Preclinical
Authors
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May advance preclinical drug safety screening; leaves open human translation and validation.
A novel high-throughput electrophysiological protocol and Markov modeling approach incorporating drug-binding kinetics significantly improves the prediction of drug-induced action potential changes for early safety assessment.
Veroli et al. (2012) studied Drug-induced QT prolongation and Torsade de Pointes risk. New electrophysiological protocol and mathematical modeling of drug-binding kinetics vs. Traditional conductance reduction methods was evaluated on Action potential predictions. A new electrophysiological protocol combined with mathematical modeling of drug-binding kinetics provided quantitative predictions of action potential modulation that were closer to experimental data.