Key result
Novel 15-second patch-clamp protocol rapidly characterizes full hERG channel kinetics and cell-to-cell variability.
Why the study?
A rapid and comprehensive protocol compatible with automated high-throughput platforms to characterize hERG channel kinetics was lacking.
A novel 15-second high-throughput patch-clamp protocol enables rapid and quantitative characterization of hERG channel kinetics, which is valuable for preclinical proarrhythmia screening.
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Facilitates rapid hERG kinetic profiling in automated screens; leaves open whether cell-specific models improve proarrhythmia risk prediction.
Lei et al. (2019) studied this question. 15 second protocol to characterize hERG kinetics was evaluated on Characterization of hERG (Kv11.1) kinetics and quantification of uncertainty. A new 15-second protocol successfully characterized full hERG channel kinetics and quantified cell-to-cell variability using a high-throughput automated patch-clamp platform.
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