Key result
Short voltage-clamp protocols consisting of steps and ramps were designed to rapidly capture hERG ion channel gating kinetics and assist with parameterisation of mathematical models.
Why the study?
The study was designed to assist with parameterisation, selection and refinement of mathematical models of hERG gating by gathering information on ion channel gating with short voltage-clamp protocols.
The proposed short voltage-clamp protocols facilitate the rapid capture of hERG kinetics for mathematical modeling and can be implemented on automated patch clamp platforms.
Short voltage-clamp protocols aid hERG model parameterization; leaves open clinical translation for arrhythmia or drug-safety prediction.
<ns3:p>We provide details of a series of short voltage-clamp protocols designed for gathering a large amount of information on hERG (Kv11.1) ion channel gating. The protocols have a limited number of steps and consist only of steps and ramps, making them easy to implement on any patch clamp setup, including automated platforms. The primary objective is to assist with parameterisation, selection and refinement of mathematical models of hERG gating. We detail a series of manual and automated model-driven designs, together with an explanation of their rationale and design criteria. Although the protocols are intended to study hERG1a currents, the approaches could be easily extended and generalised to other ion channel currents.</ns3:p>
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Lei et al. (2024) studied hERG (Kv11.1) ion channel gating. Short voltage-clamp protocols was evaluated. Short voltage-clamp protocols consisting of steps and ramps were designed to rapidly capture hERG ion channel gating kinetics and assist with parameterisation of mathematical models.
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