Key result
Recombinant Bp26 ELISA diagnoses human brucellosis with ~90% accuracy.
Why the study?
Considering the prevalence of brucellosis in Iran, it is necessary to choose a specific and sensitive laboratory method for rapid and timely diagnosis of human brucellosis.
Case-Control (n=124)
Answers are grounded in this paper and cite their sources.
Diagnostic evaluation reveals high sensitivity and accuracy for recombinant Bp26 indirect ELISA in human serum, suggesting viable utility as an alternative serological test.
Background: Considering the prevalence of brucellosis in Iran, it is necessary to choose a specific and sensitive laboratory method to diagnose it in a rapid and timely manner. The aim of this study was to assess the accuracy of indirect enzyme-linked immunosorbent assay (ELISA) for detecting brucellosis in humans in order to have an appropriate alternative to conventional tests such as Wright, 2ME, and commercial ELISA kits. Materials and Methods: In this study, the recombinant protein produced from the gene (omp28) bp26 Brucella melitensis was used as an antigen for coating microplate wells. A total of 124 serum samples of normal healthy individuals (n=62) and patients with acute brucellosis (n=62) approved by STA and 2 ME tests were entered into the study. The data were analyzed in SPSS (ver.18). Results: The mean age was 39.8±13.5 years in the patient group and 36.1±12.7 years in the healthy group. Furthermore, 66.1% of the patients were male and 62.9% lived in rural regions, while these figures were respectively 71% and 45.2% in the healthy group. The sensitivity of 92% and specificity of 87% and a positive predictive value of 88% and a negative predictive value of 92% and an accuracy of 90% were determined for ELISA kit used in this study. Conclusion: The ELISA diagnostic kit reacted to most of the positive human sera. However, this kit needs to be further evaluated with a larger sample size of clinical specimens from different regions and with various clinical forms of human brucellosis.
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Hoseini et al. (2020) conducted a case-control in Human brucellosis (n=124). Indirect ELISA prepared from recombinant Bp26 gene of Brucella melitensis vs. STA and 2 ME tests was evaluated on Diagnostic accuracy (sensitivity, specificity, PPV, NPV). An indirect ELISA using recombinant Bp26 gene of Brucella melitensis demonstrated 92% sensitivity, 87% specificity, and 90% overall accuracy for diagnosing human brucellosis.
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