Control over Enzymatic Activity by DNA‐Directed Split Enzyme Reassembly

A novel and versatile approach toward DNA-controlled enzymatic activity is presented. The approach was based on the DNA-conjugated split enzyme, murine dihydrofolate reductase (mDHFR), which can be reassembled into a catalytically active conformation by hybridization of the protein fragment conjugated oligonucleotides with a DNA template strand.