Key result
RPM-Flu v.30/31 microarray detects all 62 rhinovirus and enterovirus strains tested to species level.
Why the study?
A high confidence design method to select probes for genetically diverse viruses such as human rhinoviruses and enteroviruses was lacking for differential diagnosis of febrile respiratory illness.
The newly designed RPM-Flu v.30/31 microarray can successfully detect and serotype genetically diverse human rhinoviruses and enteroviruses using a minimal number of prototype sequences.
Validated minimal probes detect diverse HRV/HEV to species level; leaves open clinical adoption for FRI diagnosis pending prospective validation.
BACKGROUND: Febrile respiratory illness (FRI) has a high impact on public health and global economics and poses a difficult challenge for differential diagnosis. A particular issue is the detection of genetically diverse pathogens, i.e. human rhinoviruses (HRV) and enteroviruses (HEV) which are frequent causes of FRI. Resequencing Pathogen Microarray technology has demonstrated potential for differential diagnosis of several respiratory pathogens simultaneously, but a high confidence design method to select probes for genetically diverse viruses is lacking. RESULTS: Using HRV and HEV as test cases, we assess a general design strategy for detecting and serotyping genetically diverse viruses. A minimal number of probe sequences (26 for HRV and 13 for HEV), which were potentially capable of detecting all serotypes of HRV and HEV, were determined and implemented on the Resequencing Pathogen Microarray RPM-Flu v.30/31 (Tessarae RPM-Flu). The specificities of designed probes were validated using 34 HRV and 28 HEV strains. All strains were successfully detected and identified at least to species level. 33 HRV strains and 16 HEV strains could be further differentiated to serotype level. CONCLUSION: This study provides a fundamental evaluation of simultaneous detection and differential identification of genetically diverse RNA viruses with a minimal number of prototype sequences. The results demonstrated that the newly designed RPM-Flu v.30/31 can provide comprehensive and specific analysis of HRV and HEV samples which implicates that this design strategy will be applicable for other genetically diverse viruses.
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Wang et al. (2008) studied Human rhinovirus and enterovirus infections (n=62). Resequencing Pathogen Microarray RPM-Flu v.30/31 vs. Conventional sequencing and VP1 typing was evaluated on Detection and serotype identification of HRV and HEV strains. The RPM-Flu v.30/31 microarray successfully detected all 34 human rhinovirus and 28 enterovirus strains to at least the species level, and differentiated 33 HRV and 16 HEV strains to the serotype level.
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