What question did this study set out to answer?

To assess human antineuraminidase antibody responses to influenza A2 neuraminidase N2 variants using an improved hemagglutination inhibition assay and a standard enzyme inhibition test.

August 1, 1972The Journal of Immunology

Antineuraminidase Antibody Response of Man to Influenza Virus Neuraminidase N2: Results Obtained with an Improved Hemagglutination Inhibition Technique and an Enzyme Inhibition Test

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Authors

AHAlbert V. HennessyUniversity of Michigan EMElva MinuseUniversity of Michigan FDFred M. DavenportRockefeller Foundation

Discussion

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Implication

Serological analysis reveals broad antineuraminidase antibody responses in influenza A2-exposed humans, suggesting difficulties in differentiating antigenic enzymatic variants using human sera.

Key Points

To assess human antineuraminidase antibody responses to influenza A2 neuraminidase N2 variants using an improved hemagglutination inhibition assay and a standard enzyme inhibition test.
Tested serum cohorts collected between 1957 and 1968 from subjects vaccinated or naturally infected with influenza A2 strains.
Quantified antineuraminidase antibodies using recombinant viruses featuring 1957 and 1968 antigenic variants through an improved hemagglutination inhibition assay and a neuraminidase inhibition test using human serum Cohn fraction IV-4 as substrate.
The improved hemagglutination inhibition method matched the neuraminidase inhibition test in reliability for detecting antineuraminidase antibodies and identifying antibody titer increases.
Antineuraminidase antibodies were largely absent upon initial exposure to A2 strains, but antibody rises occurred with progressively higher frequency following subsequent annual exposures.
Human antineuraminidase antibody responses displayed broad cross-reactivity, complicating the serological differentiation of distinct enzymatic antigenic variants in human serum.

Cite This Study

Hennessy et al. (1972) studied this question.

synapsesocial.com/papers/6ab3314b0ffbf5071f649964 https://doi.org/10.4049/jimmunol.109.2.213

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Implication

Serological analysis reveals broad antineuraminidase antibody responses in influenza A2-exposed humans, suggesting difficulties in differentiating antigenic enzymatic variants using human sera.