Nuclear DNA Content of Basidiospores of Selected Rust Fungi as Estimated from Fluorescence of Propidium Iodide-Stained Nuclei

The relative nuclear DNA content of basidiospores of five species of Puccinia, two of Uromyces, and one of Melampsora was estimated from the fluorescence intensity of propidium iodide-stained nuclei. Relative to the nuclear fluorescence of P. graminis, taken as 1.0, other fungi had the following fluorescence values: M. lini (one collection), 2.5; P. coronata (two collections), 1.1; P. hordei (11 collections), 1.7; P. recondita (six collections), 2.0; P. sorghi (one collection), 0.8; U. appendiculatus (one collection) 6.9; U. reichertii (one collection), 1.6; and U. vignae (one collection), 4.3 (...)