Key result
Oxalate treatment localizes distinct myocardial calcium storage sites across mitochondria, sarcoplasmic reticulum, and sarcolemma.
Why the study?
The subcellular localization of calcium in mammalian heart muscle cells was not fully characterized.
This preclinical study identifies mitochondria as the primary subcellular calcium storage sites in mammalian myocardium, providing insights into excitation-contraction coupling.
Animal data map myocardial calcium sites; leaves open translation to human calcium handling or disease.
In order to detect the subcellular localization of calcium in heart muscle the treatment of myocardium with oxalate has been used.The results prove that in cardiac muscle cells in situ there are several distinct calcium storage sites: the mitochondria, the sarcoplasmic reticulum (terminal cisterns and longitudinal sarcotubules), the sarcolemmal membrane (including the intercalated discs) and the subsarcolemmal vesicles. Mitochondria are the main sites of calcium storage in the heart muscle.The functional implications of these findings in relation to excitation-contraction coupling are discussed.
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Diculescu et al. (1971) studied Normal mammalian myocardium. Potassium oxalate treatment was evaluated on Subcellular localization of calcium oxalate precipitates. Treatment of rat myocardium with oxalate revealed distinct calcium storage sites in mitochondria, sarcoplasmic reticulum, sarcolemmal membrane, and subsarcolemmal vesicles.
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