Fluorescence in situ hybridization of an ultra-small red alga, Cyanidioschyzon merolae was performed using cytoplasmic rRNA as a probe for the first time. Sonication before fixing with glutaraldehyde was useful for in situ hybridization of this alga. rRNA was localized around cell nuclei. This method can be applied for the localization of mRNA in future studies.
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Toda et al. (1996) studied this question.
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