Key result
Modified Langendorff protocol reliably boosts yields of viable adult rat cardiomyocytes.
Why the study?
Isolation of high-quantity and high-quality ventricular cardiomyocytes from adult rats remains challenging due to difficulty in producing a high yield of viable cells.
This protocol provides a reliable method for isolating high yields of viable adult rat ventricular cardiomyocytes for physiological and pharmacological studies.
May enhance rat cardiomyocyte yields for lab studies; leaves open translation to human cardiac research.
Isolation of high-quantity and high-quality ventricular cardiomyocytes from adult rats is critical to study heart physiology and pathology and for drug toxicity screening. It remains challenging to produce a high yield of viable cardiomyocytes from rats. Here, we present our modified enzymatic digestion protocol that relies on the Langendorff device to generate large numbers of viable cardiomyocytes consistently. The most critical parts of this protocol are the selection of rat age and digestion time to obtain viable cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Liu et al. (2019) and Qin et al. (2020).
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Tian et al. (2020) studied this question. Modified enzymatic digestion protocol using Langendorff device was evaluated on Yield of viable cardiomyocytes. A modified enzymatic digestion protocol relying on the Langendorff device allows for the consistent generation of large numbers of viable adult rat cardiomyocytes.
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