Improved and validated HPTLC method for quantification of oenothein B and its use for analysis ofEpilobiumangustifoliumL.

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A selective and simplest possible high-performance TLC (HPTLC) method for quantification of oenothein B on the basis of the free gallic acid and total gallic acid content after acid hydrolysis has been developed and used for analysis of cultivated Epilobium angustifolium . HPTLC of aqueous extracts of E. angustifolium was performed on silica gel with benzene-methanol-acetic acid 90:16:8 ( v/v ), as mobile phase. Quantitative evaluation of the plate was performed at 570 nm after derivatization with 1% ethanolic FeCl 3 solution. The method was validated for precision, repeatability, and accuracy. Average recovery of the active ingredient from the samples was in the range 95.43–104.57%. The calibration plots were linear in the range 440–2200 ng per band. The technique was used, for the first time, for estimation of the oenothein B content of cultivated E. angustifolium . The oenothein B content was highest at budding time (18.4–20.2 mg g −1 ) and decreased dramatically to 1.0 mg g −1 after flowering.