Key result
The gene encoding the large NFAT subunit, NF90 (ILF3), was mapped to human chromosome 19 (19q11-qter and 19p11-p13.1) using PCR amplification of human-rodent somatic cell hybrid DNAs.
The study successfully mapped the ILF3 gene, which encodes the large NFAT subunit involved in IL-2 gene regulation, to human chromosome 19.
NFAT is the key nuclear target of cyclosporine and tacrolimus for IL-2 suppression; leaves open whether selective NFAT modulators can improve outcomes.
Interleukin-2 (IL-2) is the first lymphokine secreted following T cell activation. Several transcription factors regulate IL-2 gene expression, including the nuclear factor of activated T cells (NFAT). NFAT acts at the antigen receptor response element-2 (ARRE-2) sequence in the IL-2 enhancer and is the nuclear target of T cell stimulation signals and the immunosuppressant drugs cyclosporine (Sandimmune) and FK-506 (tacrolimus), which are potent inhibitors of IL-2 gene transcription. NFAT has been cloned and found to consist of two subunits, NF45 (ILF2) and NF90 (ILF3). We have recently assigned the gene encoding the small NFAT subunit, NF45 (ILF3) to human chromosome 1 (lqll-qter and lpll-pl2). This communication reports the assignment of the gene encoding the large NFAT subunit, NF90 or interleukin enhancer binding factor 3 gene (ILF3), to human chromosome 19 (19qll-qter and 19pll-pl3.1) by polymerase chain reaction (PCR) amplification of ILF3-specific DNA sequences from well-characterized human-rodent somatic cell hybrid DNAs.
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MARCOULATOS et al. (1998) studied this question. Polymerase chain reaction (PCR) amplification was evaluated on Chromosomal assignment of the ILF3 gene. The gene encoding the large NFAT subunit, NF90 (ILF3), was mapped to human chromosome 19 (19q11-qter and 19p11-p13.1) using PCR amplification of human-rodent somatic cell hybrid DNAs.
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