Explant priming with bio-effectors improves regeneration by 6-benzylaminopurine treatment while adventitious lateral shoot development by kinetin in marine red alga Gracilaria dura (Rhodophyta)

This is the authors' abstract. We don't add key points for this paper.

The use of bio-effectors for sustainable seaweed cultivation has gained much traction lately. This study investigated the effect of priming with 6-benzylaminopurine (BAP), Kinetin (KIN), and Ascophyllum nodusum extract (AMPEP) on survival, regeneration, and adventitious lateral shoot formation in red seaweed Gracilaria dura. Treatment with varying concentrations (0.0001, 0.001, & 0.01%) and exposure times (15, 30, & 60 min) was studied. There were no statistically significant differences in AMPEP, BAP, and KIN treatments either individually or in combination on the survival of explants. Individual treatments improved regeneration (89 ± 5.48%) with BAP (0.01%, 60 min), and adventitious lateral shoot development (5.18 ± 0.84 shoots explant−1) with KIN (0.001%, 30 min) compared to controls. The effect of the combination of treatments of the bio-effectors was considerably lower. The highest regeneration (56 ± 5.48%) was reported in AMPEP (0.001%, 30 min): KIN (0.0001%, 60 min): BAP (0.1%, 60 min), while the highest adventitious lateral shoot development (2.36 ± 0.22 shoots explant−1) was reported in BAP 0.01%, 60 min: KIN (0.0001%, 60 min): AMPEP (0.001%), 30 min treatment. The study would benefit sustainable seaweed aquaculture. However, open-sea performance and agar quality studies require further evaluation.