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This study investigates the effects of benzylaminopurine and gibberellic acid on the germination and growth of broccoli seedlings.

April 15, 2026BMC Plant BiologyOpen Access

Effect of alone & combined application of benzylaminopurine and gibberellic acid on in-vitro germination and growth rate of broccoli

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Evaluation of hormonal treatments enhances growth and antioxidant properties in broccoli seedlings, suggesting improved micropropagation efficacy.

This study investigates the effects of benzylaminopurine and gibberellic acid on the germination and growth of broccoli seedlings.
Conducted five pre-sowing seed-soaking treatments with BAP and GA₃ at varying concentrations
Used Murashige and Skoog medium with 16 h photoperiod
Analyzed morphological, physiological, biochemical parameters, and gene expression via RT-qPCR
All hormonal treatments showed significantly better growth compared to the control
The combination of 25 ppm BAP and 50 ppm GA₃ yielded the highest shoot length and biomass
Antioxidant enzyme activities and transcript levels of related genes were significantly elevated under the best hormonal treatment

Khan et al. (2026) studied this question.

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Evaluation of hormonal treatments enhances growth and antioxidant properties in broccoli seedlings, suggesting improved micropropagation efficacy.