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This study aims to evaluate the effects of exogenous phytohormones on seed germination and explore the molecular responses involved.

May 22, 2026ForestsOpen Access

Effects of Exogenous Hormone Treatments on Seed Germination and Transcriptome Analysis in Zelkova schneideriana

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Randomized trial evaluates hormone effects on seed germination in Zelkova schneideriana, suggesting a basis for improved propagation.

This study aims to evaluate the effects of exogenous phytohormones on seed germination and explore the molecular responses involved.
Conducted a germination experiment with varying concentrations of hormones (BR, GA3, IAA, ABA, and 6-BA) from January to March 2024.
Assessed germination traits and performed transcriptome sequencing on seeds treated with brassinolide (BR).
Analyzed antioxidant enzyme activities and endogenous hormone levels post-treatment.
BR at 10 mg/L increased germination rate from 50% to 68% by day 40, while higher concentrations inhibited germination.
Identified 169 differentially expressed genes in BR-treated seeds linked to ROS metabolism and hormone signaling pathways.
BR treatment enhanced POD activity but reduced SOD, CAT, APX, and GR activities, indicating complex physiological responses.

Zhao et al. (2026) studied this question.

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