OMIP‐016: Characterization of antigen‐responsive macaque and human T‐cells

The present panel was optimized to assess the quality and phenotype of antigenspecific CD4 and CD8 T cells in both cynomolgus macaques and humans.The use of an identical protocol for specimens collected in the two species allows for an immediate translation of research from the macaque model to humans.Our protocol works well with cryopreserved and freshly collected PBMC.Following the fixing and permeabilization procedure, we introduced a freezing step that breaks the experimental procedure and allows the shipment of freshly stimulated and permeabilized samples to facilities equipped with instruments able to measure ten distinct fluorescences.Our procedure is thus adapted to multicenter studies where stimulation is performed on fresh PBMC and flow cytometry acquisition is done in a centralized facility.