Validation of a human-specific, multiplex platform for detecting common marmoset (Callithrix jacchus) inflammatory analytes

The common marmoset (Callithrix jacchus) is a valuable non-human primate model organism with a long history of use in biomedical research, including investigating diseases with an immune component. However, prior to this effort, there has been a lack of sufficiently validated tools to quantify inflammatory markers in the common marmoset using a multiplex approach. We provide a cross-species reactivity validation of a human-specific multiplex analysis strategy that allows for the assessment of 20 distinct inflammatory analytes in a small volume of plasma isolated from common marmoset blood. We used this novel approach to perform an assessment of the impact of sex as a biological variable on a subset of these inflammatory analytes. We found that endogenous plasma levels of the following analytes are not significantly different between female and male marmosets: CRP, ICAM-1, VCAM-1, IL-13, IL-8, MIP-1β, IFN-g, IL-4, VEGF-A, FLT-1, and bFGF. These findings demonstrate the utility of our validated method for quantifying marmoset inflammatory markers.