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The distribution of two particulate enzymes, gluconate dehydrogenase (GDH) and 2-ketogluconate dehydrogenase (2KGDH), was investigated with cell free extract through 26 strains of genus Acetobacter and genus Ghuconobacter.GDH activity was found in the cell free extracts from all strains of genus Gluconobacter and two species of genus Acetobacter, A. aceti and A. aurantium.High activity of 2KGDH was also found in the pigment-producing strains of genus Gluconobacter.Best solubilization of particulate enzymes was attained with the highest recovery when 10 mg of Triton X-100 and 30 mg of protein of particulate fractions in I ml of 0.01 M phos
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Shinagawa et al. (1976) studied this question.
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