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The aim of the present study was to compare two methods of fatty acid determination and quantifi cation, differing in the kind of solvent used.Our hypothesis stated that diethyl ether can replace dichloromethane for the extraction of fatty acids, facilitate the analysis, and reduce health hazards.The analyses were conducted on feed and duodenal digesta samples from an experiment investigating the effects of feeding increasing amounts of either rape seed or linseed oils to sheep.Based on the obtained results, we conclude that the diethyl ether method seems to be more effi cient for extraction of fatty acids from hydrolysed feed and digesta.Further investigations are needed to determine whether diethyl ether can replace dichloromethane for the extraction of fatty acids from other matrices.
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Cieślak et al. (2009) studied this question.
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