What question did this study set out to answer?

To evaluate the potential reversibility of antifertility effects on sperm quality and testicular histopathology, as well as systemic toxicity, following oral papaya seed extract administration.

October 30, 2025Tropical Journal of Natural Product ResearchOpen Access

Potential Reversibility of the Antifertility Effects of Papaya (Carica papaya) Seed Extract on Sperm Quality and Testicular Histopathology in Male Mice (Mus musculus)

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Authors

ESEviana R. Sipahutar AAAhmad Ashraf AmaliusHasanuddin University RMRahmawati MinhajatHasanuddin University

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Implication

Experimental study reveals reversible sperm reduction and testicular changes from papaya seed extract in male mice, highlighting its potential as a non-toxic male contraceptive.

Key Points

To evaluate the potential reversibility of antifertility effects on sperm quality and testicular histopathology, as well as systemic toxicity, following oral papaya seed extract administration.
Conducted a post-test-only experimental study in 25 male ICR mice (Mus musculus; aged 2–3 months, 20–30 g) assigned across five groups (n=5): control, 10% extract, 20% extract, and corresponding withdrawal groups.
Administered papaya seed extract via oral maceration for 36 consecutive days, followed by a 36-day treatment withdrawal period for the reversibility cohorts.
Evaluated sperm quality, testicular morphometry and histopathology, and liver enzyme biomarkers (AST and ALT).
Papaya seed extract significantly reduced sperm concentration in a dose-dependent manner (p < 0.05) and induced histopathological alterations, including widened interstitial spaces, seminiferous tubule dilatation, and germ cell disorganization.
Sperm quality reduction and testicular tissue abnormalities demonstrated reversibility following the 36-day treatment withdrawal, particularly in the 20% concentration group.
Testicular morphometry and serum liver enzyme levels (AST and ALT) showed no significant alterations (p > 0.05), indicating an absence of hepatic toxicity.

Cite This Study

Sipahutar et al. (2025) studied this question.

synapsesocial.com/papers/6ab3862aa7982bb6fc0e1633 https://doi.org/10.26538/tjnpr/v9i10.23

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Implication

Experimental study reveals reversible sperm reduction and testicular changes from papaya seed extract in male mice, highlighting its potential as a non-toxic male contraceptive.

Potential Reversibility of the Antifertility Effects of Papaya (Carica papaya) Seed Extract on Sperm Quality and Testicular Histopathology in Male Mice (Mus musculus)

Discussion

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Implication

Key Points

Cite This Study

Potential Reversibility of the Antifertility Effects of Papaya (Carica papaya) Seed Extract on Sperm Quality and Testicular Histopathology in Male Mice (Mus musculus)

Discussion

Member takes

Implication

Key Points

Cite This Study

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