Effect of Exposure to Bisphenol A on the Sex Differentiation in Zebrafish (Danio rerio)

The effects of bisphenol A and 17ß-estradiol on sex differentiation were investigated in the zebrafish, Danio rerio.The 20-day-old fry with undifferentiated gonads were fed with food containing bisphenol A at the dose of 500, 1000, 2000 mg⋅kg -1 and 17ß-estradiol at the dose of 20 mg⋅kg -1 diet for 45 days.Sex ratio and gonadal development after the chemical treatment were determined by histological examination of gonads.In the control group, the sex ratio of fry was 1:1 (female:male), i.e. 50% of females and 50% of males appeared.The sex of fry fed with 17ß-estradiol at the dose of 20 mg⋅kg -1 diet was all females.Feminization of the fry was induced by exogenic 17ß-estradiol.The sex of fry fed with bisphenol A at the dose of 500, 1000, 2000 mg⋅kg -1 diet was 1.4:1 (p = 0.31), 3.8:1 (p = 0.01) and 11.5:1 (p < 0.01).Bisphenol A induced feminization of the fry at the two highest doses tested.