Key result
Actinomycin D pre-treatment of human embryo lung fibroblasts halted the human cytomegalovirus replication cycle after early events, whereas other viruses were unaffected.
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The complete HCMV replication cycle requires cellular functions between early transcription and virus DNA synthesis, as demonstrated by its inhibition by actinomycin D.
The study of human cytomegalovirus (HCMV) in cultures of human embryo lung fibroblasts, pre-treated with actinomycin D, has shown that under these conditions the virus infection does not proceed beyond the 'early' events of the virus replication cycle. In the same experimental conditions the growth of poliovirus type I, vaccinia virus and herpes simplex type I virus, was completely unaffected. These results suggest that the complete HCMV replication cycle requires some cellular function(s) between early transcription of the input virus genome and virus DNA synthesis.
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Landini et al. (1979) studied Human cytomegalovirus (HCMV) infection. Actinomycin D pre-treatment vs. Other viruses (poliovirus type I, vaccinia virus, herpes simplex type I) was evaluated on Virus replication cycle progression. Actinomycin D pre-treatment of human embryo lung fibroblasts halted the human cytomegalovirus replication cycle after early events, whereas other viruses were unaffected.
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