Key result
Multiplex PCR successfully identified human coronavirus OC43 as the cause of viral pneumonia in an immunocompetent adult, enabling differentiation from COVID-19.
Case Report (n=1)
No
Multiplex PCR is a useful diagnostic tool for differentiating COVID-19 from other viral pneumonias in clinical practice.
Multiplex PCR identified HCoV-OC43 pneumonia after negative COVID RT-LAMP; hypothesis-generating for differentiation of viral pneumonias and should not yet change practice.
A 40-year-old woman developed a fever, sore throat, and cough. Coronavirus disease 2019 (COVID-19) was suspected; chest CT showed pan-lobular ground-glass opacity in the bilateral lower lobes suggesting viral pneumonia. Although a reverse transcription loop-mediated isothermal amplification (RT-LAMP) test for COVID-19 using a nasopharyngeal swab was negative, she was hospitalized and isolated because COVID-19 could not be ruled out. After admission, multiplex polymerase chain reaction (PCR) with the FilmArray Respiratory Panel 2.1 from a nasopharyngeal swab was positive for human coronavirus (HCoV) OC43. Therefore, the diagnosis was pneumonia due to HCoV-OC43. Multiplex PCR is useful for differentiating pneumonia due to COVID-19 from that due to other viral pneumonias.
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Kawataki et al. (2021) conducted a case report in Viral pneumonia (HCoV-OC43) (n=1). Multiplex Polymerase Chain Reaction (FilmArray Respiratory Panel 2.1) was evaluated on Detection of respiratory pathogen. Multiplex PCR successfully identified human coronavirus OC43 as the cause of viral pneumonia in an immunocompetent adult, enabling differentiation from COVID-19.
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