Antioxidant activity test of 70% and 96% ethanol extract of tandui stem bark (Mangifera rufocostata Kosterm.) with DPPH method

Background: The genus Mangifera has been reported to have many activities as a natural antioxidant; one of them is the tandui plant (Mangifera rufucostata Kosterm). The study of 70% and 96% ethanol extracts of tandui leaves has shown potential as an antioxidant with the DPPH method, which is a strong category. The potential as an antioxidant in leaves is also possible in other parts, such as stem bark. Objective: This study is to determine the antioxidant potential of tandui stem bark in 70% and 96% ethanol solvents with the DPPH method. Methods: The tandui stem bark was extracted using the soxhletation method with 70% and 96% ethanol solvents. Potential antioxidant activity was measured using a UV-Vis spectrophotometer and the positive control, quercetin. Results: Antioxidant activity results showed that the IC50 of 70% ethanol extract of tandui stem bark (EE70%KBT) was 9.657 µg/mL, the IC50 of 96% ethanol extract of tandui stem bark (EE96% KBT) was 6.362 µg/mL and the IC50 of positive control quercetin was 3.441 µg/mL. Conclusion: The results showed that tandui stem bark extracted using 70% and 96% ethanol has a strong potential as a natural antioxidant (very strong category) using the DPPH method.