What question did this study set out to answer?

To examine whether interaction between CD47 and its ligand SHPS-1 mediates the adhesion and morphological changes of human B lymphocytes on nonactivated endothelial cells.

April 1, 2002The Journal of Immunology

Interaction Between Src Homology 2 Domain Bearing Protein Tyrosine Phosphatase Substrate-1 and CD47 Mediates the Adhesion of Human B Lymphocytes to Nonactivated Endothelial Cells

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Authors

HYHitoshi YoshidaShowa University YTYoshiaki TomiyamaNobuhara Hospital KOKenji OritaniInternational University of Health and Welfare

Discussion

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Implication

In vitro study reveals that SHPS-1 binds CD47 to mediate B lymphocyte adhesion to nonactivated endothelial cells, indicating a steady-state recruitment mechanism.

Key Points

To examine whether interaction between CD47 and its ligand SHPS-1 mediates the adhesion and morphological changes of human B lymphocytes on nonactivated endothelial cells.
Assayed binding and adhesion of human B cell lines (including Nalm6) and tonsilar B lymphocytes using an SHPS-1-Ig fusion protein.
Tested inhibition of cell adhesion and polarization using anti-CD47 monoclonal antibodies (B6H12, SE5A5), a PI3K inhibitor (wortmannin), and pertussis toxin.
Assessed SHPS-1 expression on human tonsillar high endothelial venules and nonactivated HUVECs, and evaluated tonsilar B cell adhesion to HUVEC monolayers.
Nalm6 and tonsilar B cells adhered to immobilized SHPS-1-Ig and displayed polarization-like morphology, both of which were blocked by anti-CD47 mAbs and wortmannin, but not pertussis toxin.
Immunohistochemical staining confirmed SHPS-1 expression on macrophages and high endothelial venules in human tonsils, as well as on unstimulated HUVECs.
Adhesion of tonsilar B lymphocytes to nonactivated HUVECs was significantly inhibited by the anti-CD47 antibody SE5A5.

Cite This Study

Yoshida et al. (2002) studied this question.

synapsesocial.com/papers/6ab4837271a10e218ceb74fb https://doi.org/10.4049/jimmunol.168.7.3213

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Implication

In vitro study reveals that SHPS-1 binds CD47 to mediate B lymphocyte adhesion to nonactivated endothelial cells, indicating a steady-state recruitment mechanism.

Interaction Between Src Homology 2 Domain Bearing Protein Tyrosine Phosphatase Substrate-1 and CD47 Mediates the Adhesion of Human B Lymphocytes to Nonactivated Endothelial Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Interaction Between Src Homology 2 Domain Bearing Protein Tyrosine Phosphatase Substrate-1 and CD47 Mediates the Adhesion of Human B Lymphocytes to Nonactivated Endothelial Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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