Water Channel Protein Subtype Suggests the Origin of Renal Cell Carcinoma

No AccessJournal of UrologyInvestigative Urology1 Jul 1996Water Channel Protein Subtype Suggests the Origin of Renal Cell Carcinoma Yukio Kageyama, Sei Sasaki, Yasuko Yamamura, Hiroyuki Oshima, and Yoji Ikawa Yukio KageyamaYukio Kageyama More articles by this author , Sei SasakiSei Sasaki More articles by this author , Yasuko YamamuraYasuko Yamamura More articles by this author , Hiroyuki OshimaHiroyuki Oshima More articles by this author , and Yoji IkawaYoji Ikawa More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)66019-1AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Several subtypes of water selective channel protein have been cloned. In normal kidneys, CHIP-28 (channel forming integral protein of 28 kd) is expressed solely in the proximal tubules and descending thin limbs, and aquaporin-CD (AQP-CD) expression is restricted to collecting ducts. We assessed expression of these 2 distinct types of water channels in renal cancer tissues. Materials and Methods: Expression of CHIP-28 and AQP-CD was examined in 12 samples of primary renal cell carcinoma by Northern blot, reverse transcriptase-polymerase chain reaction and immunohistochemistry. We also evaluated expression of these 2 molecules immunohistochemically in 4 samples of Bellini duct tumor which has been though to arise from renal collecting ducts. Results: Expression of CHIP-28 was detected in all of the samples of primary renal cell carcinoma, whereas none of them expressed AQP-CD. None of 4 samples ofBellini duct tumor expressed CHIP-28, although expression of AQP-CD was recognized in 2. Conclusions: Our current observation confirms the idea of the proximal or descending tubule origin of renal cell carcinoma. References 1 : Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family. Proc. Natl. Acad. Sci. U.S.A.1991; 88: 11110. Google Scholar 2 : Cloning, characterization and chromosome mapping of human aquaporin of collecting duct. J. Clin. Invest.1994; 93: 1250. Google Scholar 3 : Isolation of human Aquaporin-CD gene. J. Biol. Chem.1994; 269: 23451. Google Scholar 4 : Developmental gene expression and tissue distribution of the CHIP-28 water-channel protein. Proc. Natl. Acad. Sci. U.S.A.1993; 90: 4500. Google Scholar 5 : Localization of the CHIP28 water channel in rat kidney. Am. J. Physiol.1992; 263: C1225. Google Scholar 6 : Cloning and expression of apical membrane water channel of rat kidney collecting tubule. Nature1993; 361: 549. Google Scholar 7 : Evolutionary conservation in the untranslated regions of actin mRNAs. Nucleic Acids Res.1984; 12: 1687. Google Scholar 8 : Analysis of a mouse monoclonal antibody that reacts with a specific region of the human proximal tubule and subsets renal cell carcinomas. Cancer Res.1989; 49: 6774. Google Scholar 9 : Renal tumors. In: . Philadelphia: W. B. Saunders Co1992: 1053. Google Scholar 10 : Transformation of human kidney proximal tubule cells by a src-containing retrovirus. Oncogene1991; 6: 2105. Google Scholar 11 : Atypical carcinoma of kidney, possibly originating from collecting duct epithelium. Urology1979; 13: 315. Google Scholar 12 : Atypical carcinoma of kidney originating from collecting duct epithelium. Urology1982; 19: 89. Google Scholar 15 : Cytomorphological typing of renal cell carcinoma-a new approach. Eur. Urol.1990; 18: 6. Google Scholar Department of Urology, the Second Department of Internal Medicine, and the Department of Retroviral Regulation, Medical Research Division, Tokyo Medical and Dental University School of Medicine, Tokyo, Japan.© 1996 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byPAUL R, NECKNIG U, BUSCH R, EWING C, HARTUNG R and ISAACS W (2018) Cadherin-6:: A New Prognostic Marker for Renal Cell CarcinomaJournal of Urology, VOL. 171, NO. 1, (97-101), Online publication date: 1-Jan-2004. Volume 156Issue 1July 1996Page: 291-295 Advertisement Copyright & Permissions© 1996 by American Urological Association, Inc.Metrics Author Information Yukio Kageyama More articles by this author Sei Sasaki More articles by this author Yasuko Yamamura More articles by this author Hiroyuki Oshima More articles by this author Yoji Ikawa More articles by this author Expand All Advertisement PDF downloadLoading ...