Key result
The NOS inhibitors L-NAME and 7-nitroindazole significantly prolonged the survival of retinal ganglion cells in rat retinal explant cultures (P<0.001), indicating neuronal NOS mediates cell death.
Why the study?
Does NOS inhibition improve survival of retinal ganglion cells in neonatal rat retinal explant cultures?
Does NOS inhibition improve survival of retinal ganglion cells in neonatal rat retinal explant cultures?
p-value: p=<0.001
Neuronal NOS-derived nitric oxide plays a crucial role in retinal ganglion cell death in rat explant cultures.
May support neuronal NOS inhibition for retinal ganglion cell survival in vitro; leaves open translation to in vivo or clinical settings.
We examined possible involvement of nitric oxide synthase (NOS) on ganglion cell death in explant cultures of neonatal rat retina. Survival of retinal ganglion cells was significantly prolonged by a broad-spectrum NOS inhibitor N(omega)-nitro-L-arginine methylester. NADPH diaphorase staining revealed a diffused distribution of NOS activity in neuropils of the inner plexiform layer as well as several neurons in the inner nuclear layer. Moreover, 7-nitroindazole but not aminoguanidine promoted the survival of retinal ganglion cells. These results suggest a crucial role of neuronal NOS-derived nitric oxide in retinal ganglion cell death.
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Katsuki et al. (2004) studied Retinal ganglion cell death. NOS inhibitors (L-NAME and 7-nitroindazole) vs. Control conditions (vehicle) was evaluated on Survival of retinal ganglion cells (p=<0.001). The NOS inhibitors L-NAME and 7-nitroindazole significantly prolonged the survival of retinal ganglion cells in rat retinal explant cultures (P<0.001), indicating neuronal NOS mediates cell death.
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