Eight microsatellite markers for South American camelids

This is the authors' abstract. We don't add key points for this paper.

Description: A small insert genomic library was prepared withllama (Lama glama) DNA cloned into pJCP1 vector, based on procedures described by Ostrander et al. 1. Construction of library and screening of clones for CA/TG repeats was carried out by Research Genetics (Huntsville, AL). Plasmids from positive clones were sequenced on ABI 377 automated DNA sequencers (Applied Biosystem, Foster City, CA) at the Veterinary Genetics Laboratory. Primer sequences and characteristics of microsatellites are presented in Table 1. Table 1. Characteristics of 8 microsatellites for South American camelids Locus Repeat motif sCloneproductize (Allelesn) rSizeange(bp) Genbank accession number Primer sequences (5′-3′) LCA56 (CA)12C(CA)2 135 16 133–169 AF091122 ATGGTGTTTACAGGGCGTTGGCATTACTGAAAAGCCCAGG LCA63 (GT)8(GC)4AC(GT)8 218 14 190–254 AF091123 TTACCCAGTCCTTCGTGGGGGAACCTCGTGGTTATGGAA LCA65 (TG)13 169 14 165–191 AF091124 TTTTTCCCCTGTGGTTGAATAACTCAGCTGTTGTCAGGGG LCA66 (CA)13 223 24 220–262 AF091125 GTGCAGCGTCCAAATAGTCACCAGCATCGTCCAGTATTCA LCA68 (GT)13 194 10 191–209 AF091126 TCCTGTCTGTGAGAAGGCTGCCGAAGGAAAAATAAAATGGAA LCA70 (TA)4(CA)11(TA)2 212 7 211–223 AF091127 TTCTGATGTATGGCATAGCGATGGGGGTAAGAGCAGGATAA LCA71 (TA)2(CA)9 137 6 136–150 AF091128 CAGACATATACCTGTATCCGTATCTATTCAGTGTTTCCTCGCAATG LCA77 (GT)3A(TG)8 235 15 233–263 AF091129 TGTTGACTAGAGCCTTTTCTTCTTTGGGCAAGAGAGACTGACTGG