Analysing the complexation of amino acids and peptides with β‐cyciodextrin using electrospray ionization mass spectrometry

The formation of non‐covalently bound inclusion complexes between ß‐cyclodextrin and peptides containing aromatic acid residues has been confirmed by elcctrospray ionization mass spectrometry. This preliminary study has indicated that phenylalanine and tryptophan residues can form 1:1 complexes with ß‐cyclodextrin whereas no evidence for such a complex could be found for tyrosine residues. Bradykinin, which contains two phenylalanine residues, formed both 1:1 and 1:2 complexes.