Effect of Resveratrol, Cinnamaldehyde and their Combinations on the Antioxidant Defense System and ATP Release of Rabbit Erythrocytes: In vitro Study

Objective: Three separate experiments were performed to evaluate the biological in vitro effects of two natural antioxidants, resveratrol (RES), cinnamaldehyde (CIN) and their combinations (RES+CIN) on rabbit erythrocytes.Materials and Methods: Suspensions of erythrocytes were divided into 15 groups (each group contains 5 replicates).In three experiments, control group was treated with phosphate buffered saline and dimethyl sulfoxide (DMSO) under the same concentration, while the phytochemical groups in the three experiments were 25, 50, 75 and 100 µM of RES or CIN or their combinations (equal mixture of RES and CIN) added to the control group, respectively and incubated for 3 h at 37EC and exposed to gentle shaking every 15 min.Results: The RES or CIN separately or in combination at different doses enhanced the activities of superoxide dismutase and catalase and improved the concentration of reduced glutathione compared to the control group.Malondialdehyde, protein carbonyl and adenosine triphosphate contents showed linear and quadratic decrease with increasing the concentration of RES or CIN or their combinations.Conclusion: The tested bioactive compounds (RES+CIN) could be helpful in improving the body cells functions without causing hazardous effects and efficiently protected the cells against oxidative injury when used at concentrations up to 100 µM and found to have synergistic effects.