The Diagnostic and Prognostic Value of Serum lncRNA NEAT1 in Colorectal Cancer

Figures

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  Figure 1. The expression level and diagnostic value of serum NEAT1 in the training cohort. (A) Serum NEAT1 was significantly increased in CRC, especially in metastatic CRC. (B) The diagnostic value of serum NEAT1 for differentiating metastatic CRC with non-metastatic CRC. (C) The diagnostic value of serum NEAT1 for identifying CRC from colorectal adenoma. (D) The diagnostic value of serum NEAT1 for identifying CRC from healthy controls. ***P<0.001.

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  Figure 2. The expression level and diagnostic value of serum NEAT1 in the validation cohort. (A) Serum NEAT1 was significantly higher in CRC, especially in metastatic CRC. (B–D) The diagnostic values of serum NEAT1 for differentiating metastatic CRC with non-metastatic CRC as well as identifying CRC from colorectal adenoma or healthy controls. ***P<0.001.

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  Figure 3. CRC cells secreted more NEAT1 into the culture media than the control cell lines. (A) The levels of NEAT1 were consistently higher in the culture media from CRC cell lines, especially from the metastatic CRC cell line SW620, than from the normal cell line. (B) The expression levels of NEAT1 was increased in the culture median of HCT116 and SW620 cells with time increased. (C–D) The expression levels of NEAT1 was increased in the culture median of HCT116 and SW620 cells with cell number increased. **P<0.01, ***P<0.001.

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  Figure 4. Increased serum NEAT1 was associated with poor overall survival of CRC. (A) Serum NEAT1 was significantly dropped in the post-operative samples. (B) For metastatic CRC cases, patients in the high serum NEAT1 suffered worse overall survival. (C) For all the CRC cases, patients with the higher serum NEAT1 level had shorter overall survival. ***P<0.001.