What question did this study set out to answer?

To characterize the mutagenic, genotoxic, and aneugenic effects of the antiparasitic drug and food preservative thiabendazole on mammalian cytogenetic endpoints.

February 17, 2006Journal of Applied Toxicology

Genotoxic and aneugenic properties of an imidazole derivative

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Authors

MCM.A. CarballoUniversity of Buenos Aires AHAdriana HickUniversity of Buenos Aires SSSonia SoloneskiConsejo Nacional de Investigaciones Científicas y Técnicas

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Implication

In vitro cytogenetic assay demonstrates thiabendazole induces chromosome damage and spindle anomalies in mammalian cells, indicating potential genotoxic and aneugenic risks.

Key Points

To characterize the mutagenic, genotoxic, and aneugenic effects of the antiparasitic drug and food preservative thiabendazole on mammalian cytogenetic endpoints.
Exposed Chinese hamster ovary cells (CHO and CHO-K1) and human peripheral blood lymphocytes to thiabendazole across a dose range up to 100 µg/ml.
Assayed sister chromatid exchange (SCE) frequencies with and without metabolic activation (S9-Mix), alongside mitotic spindle anomalies and cell proliferation kinetics.
Measured the mitotic index (MI) and replication index (RI) in cultured human peripheral blood lymphocytes.
Thiabendazole induced a significant dose-dependent increase in SCE frequency in CHO cells with S9-Mix at 50–100 µg/ml (P < 0.01) and at the highest dose without S9-Mix (P < 0.01).
Treatment at the highest concentration caused a significant increase in mitotic spindle anomalies in CHO-K1 cells (P < 0.01), reflecting disruption at the microtubule level without altering cell proliferation kinetics.
Exposure of human lymphocytes to 100 µg/ml of the drug formulation significantly decreased the mitotic index (P < 0.003) and significantly altered the replication index (P < 0.05).

Cite This Study

Carballo et al. (2006) studied this question.

synapsesocial.com/papers/6ab4da5747e1b89350b0b473 https://doi.org/10.1002/jat.1132

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Implication

In vitro cytogenetic assay demonstrates thiabendazole induces chromosome damage and spindle anomalies in mammalian cells, indicating potential genotoxic and aneugenic risks.

Genotoxic and aneugenic properties of an imidazole derivative

Discussion

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Implication

Key Points

Cite This Study

Genotoxic and aneugenic properties of an imidazole derivative

Discussion

Member takes

Implication

Key Points

Cite This Study

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