Key result
The creatine kinase (M-CK) cDNA from the mandarin fish Siniperca chuatsi was cloned, revealing high transcript expression in skin, ovary, kidney, stomach, muscle, and heart.
The study successfully cloned and characterized the M-CK cDNA from mandarin fish, revealing its structural features and tissue-specific expression patterns.
The creatine kinase (CK) cDNA from the mandarin fish Siniperca chuatsi was cloned by RT-PCR and rapid amplification of cDNA ends (RACE) methods. The structural characteristics and phylogeny of this gene were analyzed. Sequence analysis revealed a 1586 bp cDNA sequence containing 92 bp 5'-untranslated region, 348 bp 3'-untranslated region and 1146 bp open reading frame (ORF), which encoded 381 amino acids. Conserved sequence blocks of vertebrate CKs and diagnostic boxes for the muscle CK (M-CK) isozyme were identified in S. chuatsi CK. Siniperca chuatsi CK showed a higher similarity with vertebrates M-CK isozyme than other CK isozymes (Brain CK, Mitochondrial CKs) and grouped with M-CK isozyme in CK phylogeny, which strongly supported that S. chuatsi CK belongs to M-CK isozyme type. Semi-quantitative RT-PCR analysis demonstrated that the M-CK transcript expression varied among the different tissues and was detected at a high level in skin, ovary, kidney, stomach, muscle and heart, but lower in eye, brain and liver.
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Zhang et al. (2010) studied this question. Cloning and tissue expression analysis of M-CK cDNA was evaluated on M-CK transcript expression across different tissues. The creatine kinase (M-CK) cDNA from the mandarin fish Siniperca chuatsi was cloned, revealing high transcript expression in skin, ovary, kidney, stomach, muscle, and heart.
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