Key result
Amiodarone and its metabolite cause concentration-dependent hepatotoxicity and lysosomal inclusions in rat hepatocytes.
Why the study?
The hepatotoxic effects and formation of myelinoid inclusion bodies induced by amiodarone and its metabolite desethylamiodarone in hepatocytes were not fully characterized.
Do amiodarone and its metabolite desethylamiodarone induce toxicity and myelinoid inclusion bodies in cultured rat hepatocytes?
Population
Hepatocytes isolated from Sprague-Dawley rats
Comparison
Various concentrations of amiodarone vs desethylamiodarone
Design
In vitro experimental study
Follow-up
0 to 96 hr
Authors
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In vitro rat hepatocyte toxicity data with amiodarone; leaves open clinical relevance and human translation.
Do amiodarone and its metabolite desethylamiodarone induce toxicity and myelinoid inclusion bodies in cultured rat hepatocytes?
Amiodarone and its major metabolite desethylamiodarone induce lysosomal inclusions and hepatotoxicity in rat hepatocytes at concentrations only slightly higher than usual therapeutic serum levels.
Somani et al. (1990) studied this question. Amiodarone and desethylamiodarone was evaluated on Lactate dehydrogenase release and development of myelinoid inclusion bodies. Amiodarone (7.6 microM) and desethylamiodarone (8 microM) induced lysosomal inclusions and concentration-dependent toxicity in cultured rat hepatocytes after 24 to 96 hours of exposure.
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