Key result
Thidiazuron (TDZ) at 0.2 to 1.0 micromolar induced somatic embryogenesis in geranium hypocotyl explants more efficiently than N(6)-benzylaminopurine combined with indole-3-acetic acid.
Thidiazuron is highly efficient at inducing somatic embryogenesis in geranium hypocotyl explants, suggesting a novel mode of action by modulating endogenous growth regulators.
TDZ may streamline geranium propagation protocols; leaves open validation across species and commercial scales.
Somatic embryogenesis was induced in hypocotyl explants of geranium (Pelargonium x hortorum) cultured on media supplemented with various concentrations of N-phenyl-N'-1,2,3-thiadiazol-5-ylurea (thidiazuron). In less than 2 weeks, somatic embryos were observed in treatments containing levels of thidiazuron (TDZ) ranging from 0.2 to 1.0 micromolar. The use of N(6)-benzylaminopurine in combination with indole-3-acetic acid also evoked embryogenesis, but the efficiency of somatic embryo production was significantly lower than that obtained with TDZ. Hypocotyl culture for only 2 days on TDZ-supplemented medium before transfer to a basal medium was sufficient for inducing somatic embryogenesis. This distinction between the induction and expression of embryogenesis may provide an experimental system for studying the developmental biology of somatic embryogenesis. Substitution of the auxin-cytokinin requirement for the induction of somatic embryogenesis by TDZ suggests the possibility of a novel mode of its action by modulation of endogenous growth regulators.
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Visser et al. (1992) studied Somatic embryogenesis in geranium. Thidiazuron (TDZ) vs. N(6)-benzylaminopurine in combination with indole-3-acetic acid was evaluated on Somatic embryo production. Thidiazuron (TDZ) at 0.2 to 1.0 micromolar induced somatic embryogenesis in geranium hypocotyl explants more efficiently than N(6)-benzylaminopurine combined with indole-3-acetic acid.
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