Catabolism of proline is widespread in prokaryotes and in mitochondria of eukaryotic cells.In enteric bacteria, the put4 gene encodes a membrane-associated dehydrogenase (EC 1.5.99.8) that oxidizes proline to glutamate for use as the sole carbon, nitrogen or energy source (5).In addition, PutA protein also functions as the autogenous transcriptional repressor of the put operon (6, 10).The structure and function of PutA protein are interesting because it is a very large protein with multiple functions: (i) it can be isolated in both monomeric and dimeric forms (8), (ii) it has two separate enzymatic activities -proline dehydrogenase and pyrroline-5-carboxylic acid (P5C) dehydrogenase ( 8), (iii) it specifically binds put control region DNA and acts as an autogenous transcriptional repressor of the put operon (10), (iv) it binds to the cytoplasmic membrane and interacts with the electron transport chain (5), (v) it binds a number of substrates -proline, P5C, the cytoplasmic membrane, DNA, FAD, and NAD (5).In this paper we describe the complete DNA sequence of the
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Allen et al. (1993) studied this question.