Key result
An extraction-free direct RT-PCR protocol for SARS-CoV-2 detection demonstrated an overall sensitivity of 93%, reaching 100% for specimens with high to moderate viral titre (Ct <34).
Why the study?
Does a non-extraction protocol for RT-PCR maintain adequate sensitivity for detecting SARS-CoV-2 RNA in swab samples?
Population
Swab samples for SARS-CoV-2 testing
Design
Other
Authors
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May enable SARS-CoV-2 testing during reagent shortages; leaves open need for prospective validation before routine use.
Does a non-extraction protocol for RT-PCR maintain adequate sensitivity for detecting SARS-CoV-2 RNA in swab samples?
An extraction-free RT-PCR protocol provides adequate sensitivity for SARS-CoV-2 detection, offering a viable alternative during reagent supply chain failures.
Brown et al. (2020) studied SARS-CoV-2 infection. Direct RT-PCR (non-extraction protocol) was evaluated on Sensitivity for detection of SARS-CoV-2 RNA. An extraction-free direct RT-PCR protocol for SARS-CoV-2 detection demonstrated an overall sensitivity of 93%, reaching 100% for specimens with high to moderate viral titre (Ct <34).
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