Key result
RT-PCR detects human parechovirus type 4 in stool isolates with ~85% nucleotide identity.
Identified and characterized two strains of human parechovirus 4 from clinical cases in Japan.
Documents HPeV-4 in Japanese children; leaves open its clinical significance and need for broader surveillance.
Reverse transcription-PCR targeting the VP0 gene of human parechoviruses (HPeVs) was used to identify two isolates from two Japanese children's stool specimens. Molecular analysis revealed that these isolates belonged to HPeV type 4, and their nucleotide identity in the P1 region was 85.0%.
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Wakatsuki et al. (2008) studied this question. Reverse transcription-PCR identified two clinical stool isolates from Japanese children as human parechovirus type 4, sharing 85.0% nucleotide identity in the P1 region.
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