A macromolecular peptide was isolated from a culture filtrate of Strepto myces macromomyceticus n. sp. which is described in this paper.Macromomycin was isolated as a white powder by precipitation with ammonium sulfate, followed by dialysis, Dowex 1X2 column chromatography and Sephadex chromatography.It is a peptide with Sw20 value of 1.27 and a molecular weight of about 15,000.It inhibits sarcoma 180 and mouse leukemia L-1210.It inhibits S. aureus, M. flavus, S. lutea, B. subtilis, and B. anthracis.Culture nitrates of actinomycetes tested against L-1210, give much lower fre quency of positive results than when tested on Ehrlich carcinoma.However, a culture inhibiting both Ehrlich ascites carcinoma and L-1210 was found and the active agent was isolated.It is a macromolecular peptide which also inhibits the growth of Gram positive bacteria.This antibiotic was differentiated from neocar zinostatin and other known macromolecular peptides showing antitumor activity and was named macromomycin.Characteristics of the strain producing this antibiotic, processes of production and isolation, and properties of the antibiotic are reported in this paper.Characteristics of the Strain No. M480-M 1 The strain was isolated from a soil sample collected at Setagaya, Tokyo and was numbered M480-M 1. Colonies on Czapek's agar medium containing glycerol
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Chimura et al. (1968) studied this question.