Key result
Cardiac glycoside pump blockade increases Na/K pump replacement rate ~4-fold to drive functional recovery.
Why the study?
The mechanism of recovery of Na/K pump function in HeLa cells after blockade by cardiac glycosides was unclear, including the role of glycoside internalization and pump replacement.
Population
HeLa cells
Comparison
Na/K pump function before and after blockade with 1 µM ouabain or digoxin
Design
Preclinical cellular study
Authors
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May accelerate understanding of glycoside recovery in myocardium; leaves open in vivo relevance and clinical translation.
The recovery of Na/K pump function in HeLa cells after cardiac glycoside block involves accelerated internalization and replacement of sodium pumps, providing a model for myocardial detoxification.
Lamb et al. (1982) studied Na/K pump block by cardiac glycosides. Cardiac glycosides (ouabain or digoxin) was evaluated on Recovery of pump function and glycoside loss. In HeLa cells, blocking Na/K pumps with 1 µM cardiac glycosides increased the rate of pump replacement by at least 4-fold, constituting a repair process independent of direct glycoside dissociation.
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