Key result
Dihydro-ouabain inhibited the electrogenic sodium pump current in guinea-pig ventricular muscle with an equilibrium dissociation constant of 4.6 (+/-2.3) x 10(-5) M.
Population
Guinea-pig ventricular muscle preparations
Comparison
Superfusion with dihydro-ouabain (5 x 10-8 x 10… vs Resting preparation in Tyrode solution (baseline)
Design
Preclinical
Authors
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No clinical implications; leaves open translation while supporting Na/K pump electrogenicity in guinea-pig ventricle.
This preclinical study demonstrates that cardiac glycosides inhibit the electrogenic sodium pump in guinea-pig ventricular muscle, supporting a 3 Na to 2 K counter-transport mechanism.
Daut et al. (1982) studied Normal guinea-pig ventricular muscle. Dihydro-ouabain (DHO) and ouabain was evaluated on Inhibition of electrogenic pump current and binding kinetics. Dihydro-ouabain inhibited the electrogenic sodium pump current in guinea-pig ventricular muscle with an equilibrium dissociation constant of 4.6 (+/-2.3) x 10(-5) M.
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