What question did this study set out to answer?

To evaluate the catalytic capabilities of Amphitrite ornata dehaloperoxidase-hemoglobin toward monohaloindole substrates and characterize the underlying reaction mechanisms.

May 2, 2014Journal of the American Chemical SocietyOpen Access

Peroxygenase and Oxidase Activities of Dehaloperoxidase-Hemoglobin from Amphitrite ornata

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To evaluate the catalytic capabilities of Amphitrite ornata dehaloperoxidase-hemoglobin toward monohaloindole substrates and characterize the underlying reaction mechanisms.
Assayed hydrogen peroxide-dependent oxidation of 5-bromoindole using both ferric and oxyferrous states of dehaloperoxidase-hemoglobin.
Conducted oxygen isotope labeling experiments to identify the source of oxygen atoms in the resulting monooxygenated reaction products.
Analyzed aerobic reactivity, oxygen consumption, and enzyme reduction using the intermediate 5-bromo-3-oxindole under enzymatic, nonenzymatic, and anaerobic conditions.
Dehaloperoxidase-hemoglobin catalyzed the oxidation of 5-bromoindole into 5-bromo-2-oxindole and 5-bromo-3-oxindolenine, with isotope labeling confirming oxygen was derived exclusively from hydrogen peroxide.
The product precursor 5-bromo-3-oxindole reduced the ferric enzyme back to the oxyferrous globin state, establishing a product-driven pathway that restores oxygen-binding capacity.
Aerobic incubation with 5-bromo-3-oxindole in the absence of hydrogen peroxide consumed molecular oxygen to yield 5,5'-dibromoindigo, demonstrating a distinct oxidase activity.

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Implication

Biochemical study reveals novel peroxygenase and oxidase activities in Amphitrite ornata dehaloperoxidase-hemoglobin, indicating multifunctional catalytic versatility in marine globins.

Cite This Study

Barrios et al. (2014) studied this question.

synapsesocial.com/papers/6ab60c97364d4e83786fdd29 https://doi.org/10.1021/ja500293c

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Implication

Biochemical study reveals novel peroxygenase and oxidase activities in Amphitrite ornata dehaloperoxidase-hemoglobin, indicating multifunctional catalytic versatility in marine globins.

Peroxygenase and Oxidase Activities of Dehaloperoxidase-Hemoglobin from Amphitrite ornata

Key Points

Discussion

Implication

Cite This Study

Peroxygenase and Oxidase Activities of Dehaloperoxidase-Hemoglobin from Amphitrite ornata

Key Points

Discussion

Implication

Cite This Study

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