Summary A routine method giving accurate results was required for the determination of the glyceride content of eluate fractions obtained in the silicic acid chromatography of lipid mixtures. The technique of determining glyceride glycerol by saponification, oxidation of the liberated glycerol to formaldehyde, reaction of the formaldehyde with chromotropic acid and measurement of the resulting chromogen at 570 mμ has been investigated. Procedures based on this principle have appeared in the literature but none of these has been found to be entirely satisfactory. Accordingly, a modification of the method has been developed that is capable of giving results of a high degree of accuracy.
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John H. Moore (1962) studied this question.
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