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Multigram quantities of 2- and 6-nitro-3-acetamido-4-chlorobenzoic acids were separated by pH-zone-refining CCC. A two-phase solvent system composed of methyl tertiary-butyl ether, acetonitrile and water was employed using trifluoroacetic acid as a displacer acid in the organic mobile phase and ammonia as a retainer base in the aqueous stationary phase.
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Ma et al. (1994) studied this question.
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