Key result
Immunoinhibitory CK-MB assays accurately diagnose acute myocardial infarction with ~99% specificity compared to electrophoresis.
Why the study?
Two immunoinhibitory methods for measuring creatine kinase-MB needed comparison with the commonly used agarose gel electrophoretic method in suspected myocardial infarction cases.
Do immunoinhibitory methods accurately measure creatine kinase-MB compared to agarose gel electrophoresis in patients with suspected acute myocardial infarction?
Cross-Sectional (n=235)
No
Do immunoinhibitory methods accurately measure creatine kinase-MB compared to agarose gel electrophoresis in patients with suspected acute myocardial infarction?
Immunoinhibitory methods (dry and wet chemistry) for measuring CK-MB show high sensitivity and specificity compared to agarose gel electrophoresis, making them reliable for diagnosing acute myocardial infarction.
Supports interchangeability of immunoinhibitory CK-MB methods with electrophoresis; leaves open relevance versus modern troponin assays.
Two immunoinhibitory methods for measuring creatine kinase-MB (a dry chemical and a wet chemical method) were compared with the commonly used agarose gel electrophoretic method, using 563 serum samples from 235 patients with suspected acute myocardial infarction. Comparison of the electrophoretic and the dry chemistry methods showed the linear relationship: electrophoretic method = -6.5 U/l + 1.22 x dry chemistry method, r = 0.943. For the wet chemistry method the relationship was: electrophoretic method = -7.2 U/l + 1.19 x wet chemistry method, r = 0.854. Parallel determinations of total creatine kinase were also done and the methods were virtually identical in performance. Compared with the electrophoretic method (which showed a 15% prevalence of acute myocardial infarction), these classifications showed sensitivities of 0.92 and 0.67 and specificities of 0.94 and 0.99 (dry and wet chemistry, respectively; using methods recommended by the manufacturers). After optimization of discriminators the sensitivity was increased to 0.94/0.92 and the specificity to 0.99/0.99.
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Hadberg et al. (1990) conducted a cross-sectional in Suspected acute myocardial infarction (n=235). Immunoinhibitory methods (dry and wet chemistry) for CK-MB vs. Agarose gel-electrophoresis was evaluated on Diagnostic sensitivity and specificity for acute myocardial infarction. Two immunoinhibitory methods (dry and wet chemistry) for measuring CK-MB showed high correlation with agarose gel-electrophoresis and, after optimization of discriminators, achieved sensitivities of 0.94 and 0.92, respectively, with specificities of 0.99 for diagnosing acute myocardial infarction.
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