Inhibition of lipases by proteins and their inhibitory mechanism.

Inhibitory activities of proteins originated from plants and animals were estima ted for lipase. All proteins tested inhibited Rhizopus delemar and Candida cylindracea l ipases, but they did not inhibit porcine pancreatic lipase much in contrast. Homo-poly-amino acids were employed to simplify the reaction system and consequently make clear the me chanism of the lipase inhibition by proteins. Simplified interfacial studies were also p erformed to explain the inhibitory mechanism. The hydrophobicity and the conformational stru cture of each protein were concerned with the inhibitory activity. It is probable that th e protein inhibits the lipase activity by the physical separation of lipase from its subst rate.