The polyethylene glycol−sodium sulfate aqueous two-phase system has been characterized at 23 °C. Tie-lines for the phase diagram were obtained experimentally. Phases in equilibrium were characterized by means of the solvatochromic parameters π *, α, and β, which provide a measurement of the polarity/polarizability and the H-bond donor and acceptor abilities, respectively. The ability of the phases to participate in hydrophobic interactions was characterized by means of the free energy of transfer of a methylene group between the conjugated phases, using the partition of a homologous series of dinitrophenylated amino acids. The results show the effect of the presence of polymer and salt in the aqueous phase, and a comparison of both phases with pure water is made.
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Rodriguez et al. (2007) studied this question.
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